Introductory Gene Manipulation and Bioinformatics for Agriculture.
DURATION:
August 20, 2007- December 20, 2007.
TARGET GROUP:
Junior researchers in the field of molecular biology, biochemistry or genetics relating to plants.
COURSE OBJECT:
Trought the training, participants are expected to learn:
A-1.- fundamental techniques related to genetic engineering, including extraction and isolation of nucleic acids from micro-organisms and plants.
2.- manipulating DNA with restriction enzymes.
3.- identification and determination of target gene product
4.- transformation using Agrobacterium.
5.- DNA amplification with PCR.
6.- sequence determination of nucleic acids and proteins.
7.- characterization of transformed plants.
B-1.-fundamental techniques relatted to computer literacy, including:
a) webpage construction (data transfer),
b) fundamental structure of personal computers (construction, OS and software installation).
2.-Genetyx processing (gene sequence, planning)
3.-relational database processing (Access).
GENE MANIPULATION AND BIOINFORMATICS CURRICULUM
September:
· Computer skill (1) (e-mail, homepage) (Dr. WADANO)
· Outline of the course practice & Basics for Gene Manipulation (Dr. SUGIMOTO)
· Basics for Gene Manipulation (Dr. WADANO, Dr. MIURA)
· Basics for Gene Manipulation (Dr. HIBINO, Dr. TSUMURAYA)
· Preparation for Laboratory work (Dr. IWAKI)
· Library & Center for Research and Developmemt of Bioresources (Dr. INUI, Dr. IWAKI)
· Safety Procedures: Chemicals, Ultraviolet Light, Electricity, General Housekeeping
· Preparation of Solutions: Steps in Solution Preparation
· Disposal of Buffers and Chemicals
· Equipment
· Computer Skill 2 (Genetyx), (Dr. NISHIMURA) Genetyx software for many genetic analysis and databases
· Extraction and purification of nucleic acid (Bacteria), (Dr. SUMITANI, Dr. TANI)
· Computer Skill (3) (Structure of hardware) (Dr. WADANO), computer assembled.
· Transformation of E. coli (Dr. FUKADA)
· Computer Skill (4) (Preparation of individual web page), (Dr. WADANO)
October:
· Guideline for Gene Manipulation (Dr. KAWASAKI)
· PCR using Bacterial DNA (Dr. SAKAMOTO), Polimerase Chain Reaction
(PCR) technique, Electrophoresis, Picking up fragments of gel with target DNA bands, DNA extraction from agarose gels, Measure of Concentration of DNA, Results and Discussion.
· DNA sequencing (Dr. FUKADA)
· Southern Hybridization (Dr. NAKAZAWA)
· Function and application of photosynthetic microorganism (Dr. NAKANO)
· Treatment of nucleic acid with enzymes (Dr. UEDA)
· Job Report Presentation, Mid-Term Evaluation (Dr. WADANO, Dr. KAWAGUCHI)
· Introduction of genes using Agrobacterium (Dr. MISHIBA)
· SDS-PAGE (Dr. INUI Dr. IWAKI)
· Western Blotting & Affinity Purification of Recombinant Protein (Dr. YAMAJI)
November:
· Western Blotting & Affinity Purification of Recombinant Protein (Dr. YAMAJI)
· Reporter Assay (Dr. AKIYAMA)
· Preservation of Food and Agricultural Products by Irradiation (Dr. FURUTA)
· Suntory Ltd. (Dr. SHIBANO)
· Takii & Co., Ltd. (Dr. YOSHIKAWA)
· Computer Skill (5) (database) (Dr. HIBINO)
· Computer Skill (6) (database)
· Effective Utilization of Biomass (Dr. KAWAGUCHI), Introduction of genes using Agrobacterium (2) (Dr. MISHIBA)
· 20- Computer Skill (7) (database by Access) (Dr. KOYAMA Mr. KANAI)
· Analysis of Genetic Expression (Dr. IHARA)
· Cell fusion - Treatment of yeast (Dr.KISHIDA)
December:
· Introduction of genes using Agrobacterium (Dr. MISHIBA)
· Introduction of genes using particle gun (Dr. WADANO)
· Kinki University (Dr. SHIGEOKA)
· Computer Skill (8) ( blast, fasta, pdb) (Dr. TADA)
· The Present Situation and Future of GMO Plant (Dr. KOIZUMI)
· Transcriptome of rice (Dr. KITAMURA)
· Breeding of Wheats (Dr. TAIRA)
· Computer Skill (8) (Homepage Publication of Report) (Dr. WADANO)
TRAINING INSTITUTION:
Dept. Of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefectural University.
URL: http://www.osakafu-u.ac.jp/english/academics/life_envi/index.html
ACCOMMODATIONS:
JICA Osaka
URL: http://www.jica.go.jp/worldmap/english.html#osaka
